Buffered Peptone Water is rich in nutrients and produces high resuscitation rates for sub lethally injured bacteria and intense growth. The phosphate buffer system prevents bacterial damage caused by changes in the pH of the medium. Peptone including enzymatic digest of casein acts as a source of carbon, nitrogen, vitamins and minerals whilst sodium chloride maintains the osmotic balance.

Buffered Peptone Water is used in the testing of food and water samples. This product is suitable for the preliminary non selective enrichment of bacteria, particularly pathogenic Enterobacteriaceae such as Salmonella and Cronobacter, from food, animal feed, water, and other materials. The product is also suitable as a diluent for general use.

Appearance (clearness): clear

Appearance (colour): yellowish

pH-value (25 °C): 6.8 - 7.2

Growth promotion test in accordance with the current version of DIN EN ISO 11133 and the harmonised method of EP, USP and JP.

Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100

Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100

Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100

Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100

Inoculum on reference medium (Salmonella Abony NCTC 6017): ≤ 100

Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good

Growth (Escherichia coli ATCC 8739 (WDCM 00012)): good to very good

Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good

Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very good

Growth (Salmonella Abony NCTC 6017): good to very good

Incubation:

18 ± 2 hours at 37 ± 1 °C

S.abony 18 - 24 hours at 30 - 35 °C