Casein hydrolysate is commonly used in microbiology as a nitrogen source and a supplement in microbial culture media. It is produced by the digestion of casein with hydrochloric acid and contains a complex mixture of peptides and amino acids. Casein hydrolysate is particularly useful in media formulations for the growth of fastidious bacteria that require specific nutrients or additional growth factors. It provides a rich source of nitrogen, which is essential for microbial growth and protein synthesis. Casein hydrolysate can also be used as a substitute for complex peptones in certain applications, as it provides similar nutritional content.

Appearance: Lightly yellow to lightly brownishyellow fine powder.

Identity (NIR): passes test

Total nitrogen (N) (Kjeldahl): 7.0 - 8.5 %

pH-value (5 %; water): 4.7 - 7.0

Amino nitrogen (as N): 5.0 - 6.5 %

Sulfated ash (800 °C): ≤ 58 %

Loss on Drying (105°C): ≤ 6.0 %

Suitability for microbiology: passes test