GranuCult® prime Nutrient Broth is a general-purpose liquid media used for the isolation and growth of less fastidious microorganisms. It consists of peptone from meat, meat extract, yeast extract and sodium chloride. All these provide the basic nutrient requirements for the cultivation of non-fastidious microbes. The media can be enriched on the addition of supplements like blood.

GranuCult® Nutrient Broth is designed as a basic medium to support the growth of fastidious and non-fastidious microbes in test samples. It is an appropriate media for carrying out sub-culturing or to revive dormant strains.

Appearance (clearness): clear

Appearance (color): yellowish to yellowish-brown

pH-value (25 °C): 6.6 - 7.0

Growth promotion test in accordance with the current version of DIN EN ISO 11133.

Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 100

Inoculum on reference medium (Streptococcus pyogenes ATCC 12344): ≤ 100

Inoculum on reference medium (Listeria monocytogenes ATCC 19118): ≤ 100

Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≤ 100

Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100

Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100

Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≤ 100

Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): fair to very good

Growth (Streptococcus pyogenes ATCC 12344): fair to very good

Growth (Listeria monocytogenes ATCC 19118): fair to very good

Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): fair to very good

Growth (Escherichia coli ATCC 25922 (WDCM 00013)): fair to very good

Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): fair to very good

Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): fair to very good

Incubation: 24 ± 2 hours at 37 ± 1 °C, aerobic