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Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
biological source
synthetic (organic)
Quality Level
grade
Molecular Biology
description
non-ionic
form
liquid
mol wt
micellar avg mol wt 80,000
average mol wt 625
aggregation number
100-155
technique(s)
protein purification: suitable
protein quantification: suitable
western blot: suitable
impurities
<1.00% water (Karl Fischer)
pH
9.7
English
